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the elisa kit for determination of leptin  (PeproTech)


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    PeproTech the elisa kit for determination of leptin
    CERK downregulation leads to inhibition of <t>leptin</t> secretion and inhibition of PPAR- γ expression. The preadipocytes were seeded in 100 mm diameter dishes at 5 × 10 5 cells/plate and incubated for 48 h as indicated in . The cells were then electroporated in the absence of siRNA (ctrl) or in the presence of negative (scrambled) siRNA (Neg siRNA) or Cerk siRNA, as indicated in Materials and Methods. (a) Leptin concentration in supernatants was measured at day 4 after differentiation using <t>an</t> <t>ELISA</t> kit, as described in Materials and Methods. Results are the mean ± SEM of 4 independent experiments performed in duplicate ( ∗ p < 0.05). (b) PPAR γ expression was detected by Western blotting using a specific antibody. Equal loading of protein was assessed with an antibody against GAPDH. Similar results were obtained in each of 5 replicate experiments. (c) Results of scanning densitometry of the exposed film. Data are expressed as arbitrary units of intensity relative to GAPDH and are the mean ± SEM of 5 independent experiments ( ∗ p < 0.05).
    The Elisa Kit For Determination Of Leptin, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+kit+for+determination+of+leptin/human+leptin+elisa+development+kit/pmc05603748-21-6-10
    Average 90 stars, based on 1 article reviews
    the elisa kit for determination of leptin - by Bioz Stars, 2026-09
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    1) Product Images from "Implication of Ceramide Kinase in Adipogenesis"

    Article Title: Implication of Ceramide Kinase in Adipogenesis

    Journal: Mediators of Inflammation

    doi: 10.1155/2017/9374563

    CERK downregulation leads to inhibition of leptin secretion and inhibition of PPAR- γ expression. The preadipocytes were seeded in 100 mm diameter dishes at 5 × 10 5 cells/plate and incubated for 48 h as indicated in . The cells were then electroporated in the absence of siRNA (ctrl) or in the presence of negative (scrambled) siRNA (Neg siRNA) or Cerk siRNA, as indicated in Materials and Methods. (a) Leptin concentration in supernatants was measured at day 4 after differentiation using an ELISA kit, as described in Materials and Methods. Results are the mean ± SEM of 4 independent experiments performed in duplicate ( ∗ p < 0.05). (b) PPAR γ expression was detected by Western blotting using a specific antibody. Equal loading of protein was assessed with an antibody against GAPDH. Similar results were obtained in each of 5 replicate experiments. (c) Results of scanning densitometry of the exposed film. Data are expressed as arbitrary units of intensity relative to GAPDH and are the mean ± SEM of 5 independent experiments ( ∗ p < 0.05).
    Figure Legend Snippet: CERK downregulation leads to inhibition of leptin secretion and inhibition of PPAR- γ expression. The preadipocytes were seeded in 100 mm diameter dishes at 5 × 10 5 cells/plate and incubated for 48 h as indicated in . The cells were then electroporated in the absence of siRNA (ctrl) or in the presence of negative (scrambled) siRNA (Neg siRNA) or Cerk siRNA, as indicated in Materials and Methods. (a) Leptin concentration in supernatants was measured at day 4 after differentiation using an ELISA kit, as described in Materials and Methods. Results are the mean ± SEM of 4 independent experiments performed in duplicate ( ∗ p < 0.05). (b) PPAR γ expression was detected by Western blotting using a specific antibody. Equal loading of protein was assessed with an antibody against GAPDH. Similar results were obtained in each of 5 replicate experiments. (c) Results of scanning densitometry of the exposed film. Data are expressed as arbitrary units of intensity relative to GAPDH and are the mean ± SEM of 5 independent experiments ( ∗ p < 0.05).

    Techniques Used: Inhibition, Expressing, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot

    Related Articles

    other:

    Article Title: Implication of Ceramide Kinase in Adipogenesis.
    Article Snippet: The ELISA kit for determination of leptin was purchased from Peprotech.



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    CERK downregulation leads to inhibition of leptin secretion and inhibition of PPAR- γ expression. The preadipocytes were seeded in 100 mm diameter dishes at 5 × 10 5 cells/plate and incubated for 48 h as indicated in . The cells were then electroporated in the absence of siRNA (ctrl) or in the presence of negative (scrambled) siRNA (Neg siRNA) or Cerk siRNA, as indicated in Materials and Methods. (a) Leptin concentration in supernatants was measured at day 4 after differentiation using an ELISA kit, as described in Materials and Methods. Results are the mean ± SEM of 4 independent experiments performed in duplicate ( ∗ p < 0.05). (b) PPAR γ expression was detected by Western blotting using a specific antibody. Equal loading of protein was assessed with an antibody against GAPDH. Similar results were obtained in each of 5 replicate experiments. (c) Results of scanning densitometry of the exposed film. Data are expressed as arbitrary units of intensity relative to GAPDH and are the mean ± SEM of 5 independent experiments ( ∗ p < 0.05).

    Journal: Mediators of Inflammation

    Article Title: Implication of Ceramide Kinase in Adipogenesis

    doi: 10.1155/2017/9374563

    Figure Lengend Snippet: CERK downregulation leads to inhibition of leptin secretion and inhibition of PPAR- γ expression. The preadipocytes were seeded in 100 mm diameter dishes at 5 × 10 5 cells/plate and incubated for 48 h as indicated in . The cells were then electroporated in the absence of siRNA (ctrl) or in the presence of negative (scrambled) siRNA (Neg siRNA) or Cerk siRNA, as indicated in Materials and Methods. (a) Leptin concentration in supernatants was measured at day 4 after differentiation using an ELISA kit, as described in Materials and Methods. Results are the mean ± SEM of 4 independent experiments performed in duplicate ( ∗ p < 0.05). (b) PPAR γ expression was detected by Western blotting using a specific antibody. Equal loading of protein was assessed with an antibody against GAPDH. Similar results were obtained in each of 5 replicate experiments. (c) Results of scanning densitometry of the exposed film. Data are expressed as arbitrary units of intensity relative to GAPDH and are the mean ± SEM of 5 independent experiments ( ∗ p < 0.05).

    Article Snippet: The ELISA kit for determination of leptin was purchased from Peprotech.

    Techniques: Inhibition, Expressing, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot